Part one:The significance of the study, the scientific bases, research overview and trend in China and international community

1．In order to promote the development of life science industry by undertaking related projects we have to establish bioinformatics platform to serve large-scale sequencing analysis. This activity is an important action that would advance independent innovation capability in high-tech biology area of China.
　　The success of Human Genome Project brought large-scale DNA sequencing technology into mature stage and promoted the development of informatics. This practice shows that the informatization of biologic resource through large-scale DNA sequencing, including genome and cDNA, has becoming a reliable method through that we can find, separate and clone genes with highest speed and lowest cost. The informatization integrates biologic resources with genetic engineering, genetic modification technology and other molecular biology techniques and is becoming the origin and growing point of biologic industry in 21 century.
　　Up to now, we hold all key technologies used in large-scale genome studies and implement technological route which is capable of carrying out large-scale DNA sample preparing with lower cost so that we made great progress in bioinformatics area and the daily sequencing capacity exceeds 50MB. In every aspect, such as large-scale, low cost, high output and even doing information analysis in time, we are now at the same level with scientists working on the international HGP. The advanced position in international community lays the foundations for the study of biologic information resource with Chinese characteristics.
　　The biological resource specially owned by China is the important strategic resource for developing Chinese biologic industry in 21 century. Obtaining genome sequence is the only way to transfer biologic resources from colony or physical resources to biologic information, namely genome sequence. Based on systematization and informatization, biologic resources transfer will bring the exploitation and utilization of resource to an upper stage that is clear ownership and industrialization.

2．China is the nation feeding largest amount of pig in the world. Pork is the essential source from that Chinese taking protein so that pig industry plays important roll in national economy. China benefited most from the development of pig breeding science:

1） 78 million tons of pork or about one billion heads of pig were consumed every year. The output and consumption of pork in China account for 50% of the world and the production value is about 100 billion Yuan (RMB). Breeding high quality pig with higher thin-meat ratio, faster growing rate and reproduction is an important issue that Chinese agriculture development is facing.
2） In medical care and biology technology aspects, pig will become the perfect choice for human organ transplant and disease model. Since consanguinity of pig and human is so close that the size and weigh of internal organ are similar and therefore the porcine organ is the ideal material for human organ transplant. Breeding pigs with improved gene will provide the organ or cells for hetero-organ transplant after which reject reaction will not occur. This technique will have bright perspective in medical care area.
3） Over past five years, the sequencing capacity and output in the world has increased continuously by several times and bring the related industry developed quickly. As a result of industrialization, the cost sequencing per base pair DNA lowed quickly, a huge market with high profit formed, and then stimulated the need for sequencing. The production of huge amount of data promoted the development of bioinformatics and functional genomics industry that result in a developing pattern integrating genomics, technology and industry together. The most important aim of porcine genome project is to keep up with international research level and to maintain the competitive capacity of the nation.

Part two:Goal and task

The Objects in first phase

a. Objects
1） Sequence EST to get DNA sequences coding proteins;
2） In order to provide basic genome for establishing the foundation of designing and improving porcine breed, sequence genome and construct database;
3） Construct BAC library and screen out BAC clone containing important gene;
4） Identify the polymorphism of mononucleotide （SNP）of different variety of pig, search and define high grade gene, and then clarify the way and method to utilize these genes. Establish the theory foundation for constructing gene library of Chinese inherited resources of agriculture animal and information database;

b. Tasks
1） Determine at least 100 thousand EST sequences in specific library with different organs;
2） Determine 2X genome sequence, complete the working draft covering 90% genome, obtain more than 95% sequences of structural gene, establish database of genome sequence and final analysis;
3） Construct library covering at least 5X BAC, synthesize 30 thousand pairs of primers for candidate gene, screen BAC library with PCR technique to capture BAC clones containing important genes;
4） Synthesize primers for representative points, amplify and sequence DNA with PCR method and identify at least five thousand SNPs.

c. Key technological issues
1） Constructing cDNA library and sequencing cDNA or EST;
2） Drawing physical map, sequencing genome with shotgun technique;
3） Drawing the relation diagram of whole Chinese porcine breed system to identify the distribution of gene in crossbreeding process;
4） Developing the method to dope out the functions of genes and the capability of assembling sequence and finding genes.

The Objects in the second phase

1） Carry out porcine breeding by utilizing genomics and therefore improve swine production.
2） Make clinical use of genetically modified organ transplant.
3） Basic biology studies: comparing genomics, gene expression, bioinformatics, experimental zoology and other researches related to human polygenic disease, aging and health problems.
4） In order to promote the sustainable development of animal agriculture in China, we will cooperate closely with agriculture biology scientists and enterprises related to swine industry to develop the theory and technological foundation for identifying high grade livestock breeds capable of reproduce in high speed.

Perspective analysis

1） The porcine genome database itself is a product with very high commercial value.
2） SNP will become an important marker for identify genes of high-grade porcine breed and therefore provide gene resource for improving porcine breed. Improving the grade of porcine breed by using DNA recombination techniques will lead improvements of parturition rate, the daily weigh increase, thin-meet ratio, disease resistance and so on and that would be significant for economic development and raising the life standard of Chinese people.
3） Since the genes of pig and human are highly similar, the separation of functional porcine gene can be used for identifying human gene. The finding of porcine insulin gene is a good example. These genes are very valuable for industrialization.
4） The consanguinity of pig and human is so close that the size and weigh of internal organ are similar and therefore the porcine organ is the ideal material for human organ transplant. Comparing the genome of pig and human and breeding pigs with improved gene will provide the organ or cells for hetero-organ transplant after which reject reaction will not occur. This technique will have bright perspective in medical area.
5） Pig can be used as material and perfect experimental animal model for studying most often human polygenic disease and comparative genomics related to aging problem.
6） Promote the development of studying related to functional genomics and informatics.